{"id":715,"date":"2023-11-19T15:18:33","date_gmt":"2023-11-19T15:18:33","guid":{"rendered":"https:\/\/pranichealingresearch.com\/?p=715"},"modified":"2023-11-19T15:18:34","modified_gmt":"2023-11-19T15:18:34","slug":"pranic-healing-in-the-laboratory-effects-of-pranic-healing-on-calcium-release-in-immortalized-mouse-keratinocyte-hacat-cells","status":"publish","type":"post","link":"https:\/\/pranichealingresearch.com\/index.php\/2023\/11\/19\/pranic-healing-in-the-laboratory-effects-of-pranic-healing-on-calcium-release-in-immortalized-mouse-keratinocyte-hacat-cells\/","title":{"rendered":"Pranic Healing in the Laboratory, Effects of Pranic Healing on Calcium Release in Immortalized Mouse Keratinocyte HaCaT Cells"},"content":{"rendered":"\n<p><strong>Presenter:&nbsp;<\/strong>Hordep Velasquez &#8211; Caracas, Venezuela<\/p>\n\n\n\n<p><strong>Objective:&nbsp;<\/strong>This study was to observe the release of calcium into mouse keratinocyte HaCaT cells subjected to Pranic Healing.<\/p>\n\n\n\n<p><strong>Study Design:&nbsp;<\/strong>Cells were loaded with 5 \u03bcM calcium indicator Fura 2-AM to monitor changes in intracellular calcium concentration. Cell population was separated into two groups: a control group where cells received no stimulation and other experimental group where Pranic Healing was applied.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\" id=\"yui_3_17_2_1_1700407083005_99\"><img fetchpriority=\"high\" decoding=\"async\" width=\"253\" height=\"252\" src=\"https:\/\/pranichealingresearch.com\/wp-content\/uploads\/2023\/11\/image-7.png\" alt=\"\" class=\"wp-image-716\" srcset=\"https:\/\/pranichealingresearch.com\/wp-content\/uploads\/2023\/11\/image-7.png 253w, https:\/\/pranichealingresearch.com\/wp-content\/uploads\/2023\/11\/image-7-150x150.png 150w\" sizes=\"(max-width: 253px) 100vw, 253px\" \/><\/figure>\n\n\n\n<p><strong>Results:&nbsp;<\/strong>The cells that were treated with Pranic Healing show a significant increase in intracellular calcium concentration as compared with untreated cells. Such increase in calcium concentration is consistent with the depletion of intracellular stores. By the action of Thapsigargin (TG) peak calcium release is equivalent in cells exposed to Pranic Healing in comparison to control cells. One possible explanation for this observed result is that stores of intracellular calcium had been stimulated by Pranic Healing and hence the resulting drain is lower.<\/p>\n\n\n\n<p><strong>Conclusions:&nbsp;&nbsp;<\/strong>These results allow us to infer that Pranic Healing has an action on intracellular calcium storage but does not allow us to clarify how calcium has been stimulated.<\/p>\n\n\n\n<p>This study has been published.&nbsp;<a target=\"_blank\" href=\"http:\/\/www.academicpub.org\/PaperInfo.aspx?PaperID=16120\" rel=\"noreferrer noopener\"><strong>Download the Full Paper here<\/strong><\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Presenter:&nbsp;Hordep Velasquez &#8211; Caracas, Venezuela Objective:&nbsp;This study was to observe the release of calcium into mouse keratinocyte HaCaT cells subjected to Pranic Healing. Study Design:&nbsp;Cells were loaded with 5 \u03bcM calcium indicator Fura 2-AM to monitor changes in intracellular calcium concentration. Cell population was separated into two groups: a control group where cells received no [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":692,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[9],"tags":[],"class_list":["post-715","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-2015-conference-in-bogota"],"_links":{"self":[{"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/posts\/715","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/comments?post=715"}],"version-history":[{"count":1,"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/posts\/715\/revisions"}],"predecessor-version":[{"id":717,"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/posts\/715\/revisions\/717"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/media\/692"}],"wp:attachment":[{"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/media?parent=715"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/categories?post=715"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pranichealingresearch.com\/index.php\/wp-json\/wp\/v2\/tags?post=715"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}